Novel zwitterionic HILIC stationary phase for the determination of ethyl glucuronide in human hair by LC-MS/MS
作者: Diego PalumboPaolo FaisAnna CalìMonica LusardiElisabetta BertolJennifer P. Pascali
作者单位: 1Forensic Toxicology Division, Department of Health Sciences, University of Florence, Largo Brambilla 3, 50134 Florence, Italy
2University of Bologna, Department of Medical and Surgical Sciences, Unit of Legal Medicine, Via Irnerio 49, Bologna, Italy
3Agilent Technologies, Via V. Lamaro, Rome, Italy
刊名: Journal of Chromatography B, 2018, Vol.1100-1101 , pp.33-38
来源数据库: Elsevier Journal
DOI: 10.1016/j.jchromb.2018.09.027
关键词: Ethyl glucuronideLiquid chromatographyHairHydrophilic chromatographyAlcoholHILIC-Z
英文摘要: Abstract(#br)Some recent studies have described a shift from traditional reversed-phase to more hydrophilic LC chemistry for EtG determination in hair (hEtG). The reason relies on the poor retention of C8– and C18-based columns for polar compounds, even in presence of great amount of aqueous phase. This work presents the development, validation and application of a new hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-LC-MS/MS) method based on a novel zwitterionic stationary phase for the analysis of hEtG. The linearity was assessed in the range of 5–100 pg/mg hair, with a correlation coefficient of >0.99. The method was selective and sensitive, with a limit of detection (LOD) and limit of quantitation (LOQ) of 1.4 pg/mg and 4.5 pg/mg in hair, respectively....
全文获取路径: Elsevier  (合作)
影响因子:2.487 (2012)

  • glucuronide 葡糖苷酸
  • determination 测定
  • zwitterionic 两性离子的
  • chromatography 色层分离法
  • human 人的
  • phase 相位
  • stationary 固定的
  • Alcohol 酒精
  • ethyl 乙基