Molecular cloning and characterization of LrTLR4, analysis of its inductive expression and associated down-stream signaling molecules following lipopolysaccharide stimulation and Gram-negative bacterial infection
作者: Mrinal SamantaMadhubanti BasuBanikalyan SwainMahismita PaichhaSaswati S. LenkaSurajit DasPallipuram JayasankarNikhil Kumar Maiti
作者单位: 1Immunology Laboratory, Fish Health Management Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, India
2Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela 769 008, Odisha, India
刊名: Fish and Shellfish Immunology, 2017, Vol.60 , pp.164-176
来源数据库: Elsevier Journal
DOI: 10.1016/j.fsi.2016.11.028
关键词: Labeo rohitaTLR4Innate immunityIL-6IL-8Type I IFN
英文摘要: Abstract(#br)Toll-like receptors (TLRs) play key roles in innate immunity from lower to higher vertebrates. Among various TLR types, TLR4 was reported to recognize LPS in higher vertebrates resulting in the activation of down-stream signaling pathway. Except in some teleosts, function of TLR4 in most fish species including rohu ( Labeo rohita ) a commercially important fish species in the South-East Asian countries remained unknown. To investigate it, full-length cDNA of Labeo rohita TLR4 (LrTLR4) was cloned, and it consisted of 2729 bp, with a single ORF of 2469 bp encoding a polypeptide of 822 aa with a predicted molecular mass of 94.753 kDa. Structurally, LrTLR4 consisted of 25 LRRs (leucine rich repeat regions), one TM (trans-membrane) domain and one TIR (Toll/interleukin-1 receptor)...
全文获取路径: Elsevier  (合作)
影响因子:2.964 (2012)

  • expression 表示
  • bacterial 细菌的
  • negative 负象
  • cloning 无性繁殖系化
  • inductive 电感的
  • stimulation 激励
  • analysis 分析
  • associated 相关的
  • stream 
  • signaling 发信号