Performance evaluation of anti-fixed Leishmania infantum promastigotes immunoglobulin G (IgG) detected by flow cytometry as a diagnostic tool for visceral Leishmaniasis
作者: Elis D. SilvaBeatriz C. OliveiraAndresa P. OliveiraWagner J.T. SantosGeorge T. DinizOsvaldo P. de Melo NetoCarlos H.N. CostaMauro R.B. SilvaLuiz D. AndradeZulma M. MedeirosValéria R.A. Pereira
作者单位: 1Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Instituto Aggeu Magalhães, Recife, Pernambuco, Brazil
2Instituto de Doenças Tropicais Natan Portella (IDTNP), Teresina, Piaui, Brazil
3Universidade Estadual do Piauí, Teresina, Piaui, Brazil
4Faculdade de Ciências Médicas, Universidade de Pernambuco, Recife, Brazil.
刊名: Journal of Immunological Methods, 2019, Vol.469 , pp.18-25
来源数据库: Elsevier Journal
DOI: 10.1016/j.jim.2019.02.009
关键词: Flow cytometryIgGSerodiagnosisLeishmania infantumVisceral leishmaniasis
英文摘要: Abstract(#br)Visceral Leishmaniasis (VL) is a severe disease, caused by the protozoans Leishmania infantum and L. donovani that is widely diagnosed using serological tools. These, however, have limitations in performance that limit their use for the correct identification of the cases. This study aimed to evaluate the performance of flow cytometry with fixed parasites for VL diagnosis, comparing it with four other serological tests. Samples from two endemic VL regions in Brazil, diagnosed by direct examination (DG1) and by at least two or one standard serological test (DG2 and DG3, respectively), as well as patients with chronic Chagas' disease (CG1) and healthy controls (CG2) were used in this study. The flow cytometry results were expressed as levels of IgG reactivity, based on the...
全文获取路径: Elsevier  (合作)
影响因子:2.225 (2012)

  • Leishmania 利什曼虫
  • IgG 免疫球蛋白 G
  • immunoglobulin 免疫球蛋白
  • leishmaniasis 利什曼病
  • cytometry 血细胞计数
  • detected 找到
  • diagnostic 诊断的
  • visceral 内脏的
  • fixed 固定
  • evaluation 评价