|作者：||Elis D. Silva, Beatriz C. Oliveira, Andresa P. Oliveira, Wagner J.T. Santos, George T. Diniz, Osvaldo P. de Melo Neto, Carlos H.N. Costa, Mauro R.B. Silva, Luiz D. Andrade, Zulma M. Medeiros, Valéria R.A. Pereira|
1Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Instituto Aggeu Magalhães, Recife, Pernambuco, Brazil
2Instituto de Doenças Tropicais Natan Portella (IDTNP), Teresina, Piaui, Brazil
3Universidade Estadual do Piauí, Teresina, Piaui, Brazil
4Faculdade de Ciências Médicas, Universidade de Pernambuco, Recife, Brazil.
|刊名：||Journal of Immunological Methods, 2019, Vol.469 , pp.18-25|
|关键词：||Flow cytometry; IgG; Serodiagnosis; Leishmania infantum; Visceral leishmaniasis;|
|英文摘要：||Abstract(#br)Visceral Leishmaniasis (VL) is a severe disease, caused by the protozoans Leishmania infantum and L. donovani that is widely diagnosed using serological tools. These, however, have limitations in performance that limit their use for the correct identification of the cases. This study aimed to evaluate the performance of flow cytometry with fixed parasites for VL diagnosis, comparing it with four other serological tests. Samples from two endemic VL regions in Brazil, diagnosed by direct examination (DG1) and by at least two or one standard serological test (DG2 and DG3, respectively), as well as patients with chronic Chagas' disease (CG1) and healthy controls (CG2) were used in this study. The flow cytometry results were expressed as levels of IgG reactivity, based on the... percentage of positive fluorescent parasites (PPFP). Using a 1:4096 serum dilution, a ROC curve analysis of the serum titration on flow cytometry has indicated a PPFP of 2% as the cutoff point to segregate positive and negative results. In the present study, flow cytometry had the best performance for DG1 (sensitivity of 96%) while rK39 (imunocromagraphic rapid test) and DAT (Direct agglutination test) were also associated with high sensitivity and specificity. The substantial agreement and kappa indexes observed suggested similar performances between these two tests and flow cytometry. IFAT (Immunofluorescent antibody test) and ELISA (Enzyme-linked immunosorbent assay) had lower performances and the lower values of agreement with flow cytometry. Together, these findings suggest that although adjustments are needed in order to reduce cross reactivity with other trypanosomatids, flow cytometry has the potential to be a safe serological alternative for the diagnosis of VL.|