Regulation of the sperm EGF receptor by ouabain leads to initiation of the acrosome reaction
作者: Limor DanielNir EtkovitzShirel Rosenwasser WeissSara RubinsteinDebby IckowiczHaim Breitbart
作者单位: 1The Mina and Everard Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
刊名: Developmental Biology, 2010, Vol.344 (2), pp.650-657
来源数据库: Elsevier Journal
DOI: 10.1016/j.ydbio.2010.05.490
关键词: Acrosome reactionCalciumKinasesSignal transductionSperm capacitationOuabain
原始语种摘要: Abstract(#br)The sperm acrosome reaction occurs after the binding of the capacitated sperm to the egg zona pellucida. This study describes a novel mode of regulation of the sperm epidermal growth factor receptor (EGFR) under physiological conditions and its relevance to the acrosome reaction. Ouabain, a known Na/K ATPase blocker is present in the blood and in the female reproductive tract. We show here that physiological concentrations (nM) of ouabain enhance phosphorylation of EGFR on tyr-845, stimulate Ca 2+ influx and induce the acrosome reaction in sperm. These effects could be seen only in the presence of very low concentrations of EGF (0.1ng/ml or 0.016 nM) added together with nano-molar ouabain. Phosphorylation, Ca 2+ influx, and the acrosome reaction are...
全文获取路径: Elsevier  (合作)
影响因子:3.868 (2012)

  • acrosome 顶体
  • sperm 精子
  • ouabain 哇巴因
  • reaction 反酌
  • capacitation 获能
  • receptor 接受体
  • EGF 表皮生长因子
  • leads 引线
  • transduction 转导
  • concentrations 浓聚物