In vivo expression of a short peptide designed from late embryogenesis abundant protein for enhancing abiotic stress tolerance in Escherichia coli
作者: Nishit PathakShinya Ikeno
作者单位: 1Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu 808-0196, Japan
2Research Center for Bio-microsensing Technology (RCBT), Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu 808-0196, Japan
刊名: Biochemical and Biophysical Research Communications, 2017
来源数据库: Elsevier Journal
DOI: 10.1016/j.bbrc.2017.08.091
关键词: Escherichia coliLEA peptideAbiotic stress toleranceLate embryogenesis abundant protein
英文摘要: Abstract(#br)In vivo functional analyses of a late embryogenesis abundant (LEA) short peptide expressed in recombinant Escherichia coli BL21 (DE3) were carried out under abiotic stress (salt, heat, and cold) conditions. Our LEA peptide was derived from the Polypedilum vanderplanki group 3 LEA protein based on distinctive conserved amino acid motif sequences. We focused on high-salt (5% and 7% NaCl) concentrations to evaluate the functional relevance of the peptide under abiotic salt stress. E. coli transformants expressing the LEA peptide showed higher cell viability than the control not expressing the peptide when transferred to a medium containing 5% and 7% NaCl; cells expressing LEA peptide showed a higher number of colony-forming units per dilution under the high salt stress...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • abundant 丰富的
  • embryogenesis 胚发生
  • expression 表示
  • Escherichia 埃希氏菌属
  • designed 设计了的计划了的
  • protein 蛋白质
  • abiotic 非生物的
  • enhancing 增强
  • short 短的
  • stress 应力