Construction of a synthetic metabolic pathway for the production of 2,4-dihydroxybutyric acid from homoserine
作者: Thomas WaltherFlorence CalvayracYoann MalbertCeren AlkimClémentine DressaireHélène CordierJean Marie François
作者单位: 1LISBP, Université de Toulouse, CNRS, INRA, INSA, 135 Avenue de Rangueil, F-31077 Toulouse, France
2TWB, 3 rue Ariane, 31520 Ramonville-St. Agne, France
刊名: Metabolic Engineering, 2018, Vol.45 , pp.237-245
来源数据库: Elsevier Journal
DOI: 10.1016/j.ymben.2017.12.005
原始语种摘要: Abstract(#br)2,4-dihydroxybutyrate (DHB) is a precursor for the chemical synthesis of the methionine analogue 2-hydroxy-4-(methylthio)butyrate. Since no annotated metabolic pathway exists for its microbial production from sugar, we have conceived a two-step synthetic metabolic pathway which converts the natural amino acid homoserine to DHB. The pathway proceeds through the homoserine transaminase-catalyzed deamination of homoserine to obtain 2-oxo-4-hydroxybutyrate (OHB), and continues with the reduction of OHB to DHB, which is catalyzed by an OHB reductase enzyme. We identified homoserine transaminase and OHB reductase activity in several candidate enzymes which act on sterically cognate substrates, and improved OHB reductase activity of lactate dehydrogenase A of Lactococcus lactis by...
全文获取路径: Elsevier  (合作)
影响因子:6.859 (2012)

  • 脱氨基酌 酶类
  • homoserine 高丝氨酸
  • pathway 轨道
  • enzymes 酶类
  • deamination 酶类
  • reductase 还原酶
  • transaminase 转氨酶
  • synthetic 合成的
  • cognate 同源的
  • production 生产
  • Escherichia 埃希氏菌属