Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein
大肠杆菌对紫外线的耐受性通过体内表达的一种短肽设计自胚胎发生后期丰富的蛋白
作者: Alaa HuwaidiNishit PathakAmir SyahirShinya Ikeno
作者单位: 1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Kitakyushu, Fukuoka, Japan
刊名: Biochemical and Biophysical Research Communications, 2018, Vol.503 (2), pp.910-914
来源数据库: Elsevier Journal
DOI: 10.1016/j.bbrc.2018.06.095
关键词: Escherichia coliShort peptideUltraviolet radiationLate embryogenesis abundant protein
英文摘要: Abstract(#br)Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield that protects macromolecules from various abiotic stress, was obtained from the Polypedilum vanderplanki group 3 LEA protein. Recombinant Escherichia coli BL21 (DE3) expressing functional LEA short peptide in vivo were exposed to UVA and UVC radiation for 4, 6, and 8 h. E. coli transformants expressing the LEA peptide showed higher cell viability under both UVA and UVC treatment at all time points as compared with that of the control. Furthermore, the cells expressing LEA peptide...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • abundant 丰富的
  • embryogenesis 胚发生
  • expression 表示
  • Escherichia 埃希氏菌属
  • designed 设计了的计划了的
  • ultraviolet 紫外线的
  • protein 蛋白质
  • radiation 辐射
  • short 短的
  • tolerance 耐性