Aggregation effects of gold nanoparticles for single-base mismatch detection in influenza A ( H1N1 ) DNA sequences using fluorescence and Raman measurements
作者: Erdene-Ochir GanboldTaegyeong KangKangtaek LeeSo Yeong LeeSang-Woo Joo
作者单位: 1Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea
2Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul 120-749, Republic of Korea
3Department of Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea
刊名: Colloids and Surfaces B: Biointerfaces, 2012, Vol.93 , pp.148-153
来源数据库: Elsevier Journal
DOI: 10.1016/j.colsurfb.2011.12.026
关键词: DNA hybridizationSingle nucleotide polymorphismGold nanoparticlesFluorescence quenchingSurface-enhanced Raman scatteringH1N1 virus
原始语种摘要: Abstract(#br)Aggregation effects of gold nanoparticles (AuNPs) were examined for the discrimination of single point mutations through the hybridization of oligonucleotides (25–50nM) modified with a fluorescent Texas red dye. The sequences of oligonucleotides were designed to detect the H1N1 virus gene. Single-base mismatch detection due to different adsorption propensities of oligonucleotides could be achieved using fluorescence quenching and surface-enhanced Raman scattering (SERS) properties of the dye. We observed that the addition of perfectly matched double stranded DNA (pmdsDNA), modified with the Texas red dye in the suspension of citrate-reduced AuNPs could increase fluorescence recovery intensities more substantially than either single-base mismatched double...
全文获取路径: Elsevier  (合作)
影响因子:3.554 (2012)

  • DNA Deoxyribonucleic Acid
  • fluorescence 荧光
  • mismatch 不匹配
  • stranded 绞合的搁浅的
  • nucleotide 核苷酸
  • single 单独的
  • observed 观察到的
  • hybridization 混合酌
  • fluorescent 萤光的
  • detection 探测