Control of the Flow Properties of DNA by Topoisomerase II and Its Targeting Inhibitor
作者: Binu KundukadJohan R.C. van der Maarel
作者单位: 1Biophysics and Complex Fluids Group, Department of Physics, National University of Singapore, Singapore
刊名: Biophysical Journal, 2010, Vol.99 (6), pp.1906-1915
来源数据库: Elsevier Journal
DOI: 10.1016/j.bpj.2010.07.013
原始语种摘要: Abstract(#br)The flow properties of DNA are important for understanding cell division and, indirectly, cancer therapy. DNA topology controlling enzymes such as topoisomerase II are thought to play an essential role. We report experiments showing how double-strand passage facilitated by topoisomerase II controls DNA rheology. For this purpose, we have measured the elastic storage and viscous loss moduli of a model system comprising bacteriophage λ -DNA and human topoisomerase II α using video tracking of the Brownian motion of colloidal probe particles. We found that the rheology is critically dependent on the formation of temporal entanglements among the DNA molecules with a relaxation time of ∼1 s. We observed that topoisomerase II effectively removes these entanglements and transforms...
全文获取路径: Elsevier  (合作)
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来源刊物:
影响因子:3.668 (2012)

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关键词翻译
关键词翻译
  • DNA Deoxyribonucleic Acid
  • bacteriophage 噬菌体
  • strand 海滨
  • observed 观察到的
  • elasticity 弹性
  • temporal 现世的
  • indirectly 间接地
  • viscous 粘滞的
  • formation 建造
  • topoisomerase 拓扑异构酶