Colorimetric detection of mutations in epidermal growth factor receptor using gold nanoparticle aggregation
作者: Hosub LeeTaegyeong KangKyong-Ah YoonSo Yeong LeeSang-Woo JooKangtaek Lee
作者单位: 1Department of Chemical and Biomolecular Engineering, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul, Republic of Korea
2National Cancer Center, Goyang, Gyeonggi, 410-769, Republic of Korea
3Laboratory of Veterinary Pharmacology, Seoul National University, Seoul, Republic of Korea
4Department of Chemistry, Soongsil University, Seoul, Republic of Korea
刊名: Biosensors and Bioelectronics, 2010, Vol.25 (7), pp.1669-1674
来源数据库: Elsevier Journal
DOI: 10.1016/j.bios.2009.12.002
关键词: Non-small cell lung cancerEpidermal growth factor receptor (EGFR)MutationGold nanoparticleAggregationColorimetric sensor
原始语种摘要: Abstract(#br)We have detected mutations in the epidermal growth factor receptor (EGFR) of non-small cell lung cancer cells using the selective aggregations of gold nanoparticles. Mutations in exon 19 and exon 21 of EGFR gene were detected in non-amplified genomic DNAs that were isolated from both the lung cancer cell lines and the cancer tissues of non-small cell lung cancer patients. At the optimal salt concentration, addition of the mutant DNA that was hybridized with the complementary probe into the suspension of unmodified gold nanoparticles caused the substantial aggregation of the gold nanoparticles and the color change of solution. Gold nanoparticles, however, did not exhibit significant aggregation, and the solution color remained unchanged with the addition of the wild type DNA...
全文获取路径: Elsevier  (合作)
影响因子:5.437 (2012)

  • nanoparticle 乳酸
  • amplified verification扩大核查
  • factor 因素
  • growth 生长
  • small 小的
  • genomic 染色体组的
  • receptor 接受体
  • aggregation 聚集
  • complementary 补的
  • addition 添加