Development of loop-mediated isothermal amplification (LAMP) as a diagnostic tool of toxoplasmosis
作者: D. KrastevaM. ToubianaSri HartatiAsmarani KusumawatiJ.F. DubremetzJ. Sri Widada
作者单位: 1UMR 5119, CNRS-UM2, Université Montpellier II, 34095 Montpellier Cedex 05, France
2Faculty of Veterinary Medicine, University Gadjah Mada, Yogyakarta 55281, Indonesia
3UMR CNRS 5235, Université Montpellier II, 34095 Montpellier Cedex 05, France
刊名: Veterinary Parasitology, 2009, Vol.162 (3-4), pp.327-331
来源数据库: Elsevier Journal
DOI: 10.1016/j.vetpar.2009.03.014
关键词: DiagnosisLAMPPCRSAG1Toxoplasma gondii
原始语种摘要: Abstract(#br)Infection with Toxoplasma gondii is one of the most common parasitic infections in humans and other warm-blooded animals. This paper describes the development of loop-mediated isothermal amplification (LAMP) specific to the single-copy gene SAG1 as a diagnostic tool of toxoplasmosis. A set of primers, composed of outer primers, inner primers and loop primers was designed from a published sequence data (GeneBank Acc. no. AY651825 ). Experiments showed that when LAMP was applied to sample organs, amplification absolutely required the loop primers to complete. SAG1-based LAMP turned out to be very sensitive, exhibiting a degree of sensitivity higher than the conventional PCR. LAMP is a convenient and sensitive diagnostic tool for routine health control of toxoplasmosis.
全文获取路径: Elsevier  (合作)
影响因子:2.381 (2012)

  • 公布的 方便的
  • toxoplasmosis 弓形体病
  • LAMP Library Addition and Maintenance Point
  • diagnostic 诊断的
  • amplification 放大
  • isothermal 等温的
  • parasitic 寄生的
  • routine 程序
  • convenient 方便的
  • published 方便的
  • designed 设计了的计划了的