Significantly enhanced substrate tolerance of Pseudomonas putida nitrilase via atmospheric and room temperature plasma and cell immobilization
作者: Ting-Ting DongJin-Song GongBing-Chen GuQiang ZhangHeng LiZhen-Ming LuMao-Lin LuJin-Song ShiZheng-Hong Xu
作者单位: 1School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China
2National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China
3Jiangsu Institute of Microbiology, Wuxi 214063, PR China
刊名: Bioresource Technology, 2017, Vol.244 , pp.1104-1110
来源数据库: Elsevier Journal
DOI: 10.1016/j.biortech.2017.08.039
关键词: ARTPImmobilizationNicotinic acidNitrilaseSubstrate tolerance
英文摘要: Abstract(#br)The objective of the study was to enhance the substrate tolerance of Pseudomonas putida nitrilase via atmospheric and room temperature plasma (ARTP) and cell immobilization. The mutant library was constructed by ARTP and rapidly screened by an OPA-TCA microscale reaction. A mutant strain of mut-D3 was obtained and its optimum substrate concentration was improved to 150mM from 100mM. It could accumulate 189g/L nicotinic acid (NA) from 3-cyanopyridine (3-CP), which was increased by 42% compared with that of wild type (WT). Additionally, composite immobilization of mut-D3 was performed and SA-PVA immobilized cells could catalyze 250mM 3-CP each batch with finally accumulating 346g/L...
全文获取路径: Elsevier  (合作)
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来源刊物:
影响因子:4.75 (2012)

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关键词翻译
关键词翻译
  • nitrilase 腈水解酶
  • temperature 温度
  • substrate 基质
  • plasma 深绿玉髓
  • tolerance 耐性
  • atmospheric 大气的
  • immobilization 固定
  • Substrate 感光胶层、基片
  • enhanced 增强