Near infrared lasers in flow cytometry
作者: William G. Telford
作者单位: 1Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
刊名: Methods, 2015, Vol.82 , pp.12-20
来源数据库: Elsevier Journal
DOI: 10.1016/j.ymeth.2015.03.010
关键词: Flow cytometryLaserNear-infraredAlexa Fluor dyes
英文摘要: Abstract(#br)Technology development in flow cytometry has closely tracked laser technology, the light source that flow cytometers almost exclusively use to excite fluorescent probes. The original flow cytometers from the 1970s and 1980s used large water-cooled lasers to produce only one or two laser lines at a time. Modern cytometers can take advantage of the revolution in solid state laser technology to use almost any laser wavelength ranging from the ultraviolet to the near infrared. Commercial cytometers can now be equipped with many small solid state lasers, providing almost any wavelength needed for cellular analysis.(#br)Flow cytometers are now equipped to analyze 20 or more fluorescent probes simultaneously, requiring multiple laser wavelengths. Instrument developers are now trying...
全文获取路径: Elsevier  (合作)
影响因子:3.641 (2012)

  • infrared 红外线
  • cytometry 血细胞计数
  • Laser 莱塞牌汽车