Efficient biocatalytic synthesis of nicotinic acid by recombinant nitrilase via high density culture
作者: Jin-Song GongQiang ZhangBing-Chen GuTing-Ting DongHeng LiHui LiZhen-Ming LuJin-Song ShiZheng-Hong Xu
作者单位: 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China
2National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China
刊名: Bioresource Technology, 2018, Vol.260 , pp.427-431
来源数据库: Elsevier Journal
DOI: 10.1016/j.biortech.2018.03.109
关键词: BiocatalysisNitrilaseNicotinic acidHigh density cultureFed-batch conversion
原始语种摘要: Abstract(#br)The constitutively expression system for P. putida nitrilase was firstly constructed to improve the nicotinic acid production and reduce the production costs. High density culture strategy was employed to enhance the biomass and nitrilase production of recombinant strain. The total nitrilase activity reached up to 654 U·mL −1 without the induction. 541 g·L −1 nicotinic acid was accumulated via fed batch mode of substrate feeding through 290 min of conversion.
全文获取路径: Elsevier  (合作)
影响因子:4.75 (2012)

  • density 密度
  • culture 
  • nitrilase 腈水解酶
  • batch 一批
  • nicotinic 烟碱酸的
  • recombinant 重组体
  • synthesis 合成
  • conversion 转换