Gene analysis of the N-terminal region of the estrogen receptor alpha in preeclampsia
作者: Ariadne Malamitsi-PuchnerJohn TziotisDimitrios EvangelopoulosLazaros FountasGeorge VlachosGeorge CreatsasConstantine E SekerisParaskevi Moutsatsou
作者单位: 12nd Department of Obstetrics and Gynecology, University of Athens, Athens, Greece
2Department of Biological Chemistry, University of Athens, Athens, Greece
31st Department of Obstetrics and Gynecology, University of Athens, Athens, Greece
刊名: Steroids, 2001, Vol.66 (9), pp.695-700
来源数据库: Elsevier Journal
DOI: 10.1016/S0039-128X(01)00101-5
关键词: SteroidsEstrogen receptor alphaPreeclampsiaHypertensionPregnancy
原始语种摘要: Abstract(#br)Alterations in the NH 2 -terminal region of the estrogen receptor alpha (ERα) gene expressed in placental bed tissue may be implicated in the development of preeclampsia, the pathogenesis of which involves the spiral arteries. Therefore, mutations and polymorphisms on exons 1 and 2 of the gene encoding ERα were studied. Placental bed biopsies were taken from 20 healthy, normotensive pregnant women and 16 preeclamptic patients. DNA was extracted from the tissue and exon 1 and exon 2 were amplified by PCR prior to denaturing gradient gel electrophoresis analysis or to single stranded conformational polymorphism analysis. In exon 1, a codon 10 polymorphism, either homozygous for the wild type gene, homozygous for the mutant type gene, or heterozygous, was revealed in both...
全文获取路径: Elsevier  (合作)
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影响因子:2.803 (2012)

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关键词翻译
关键词翻译
  • region 地域
  • preeclampsia 子痫前期
  • analysis 分析
  • receptor 接受体
  • homozygous 纯合的
  • alpha 接字母顺序的
  • amplified verification扩大核查
  • terminal 顶端的
  • estrogen 雌激素
  • either 任何