The effects of sub-lethal concentrations of silver nanoparticles on inflammatory and stress genes in human macrophages using cDNA microarray analysis
作者: Dae-Hyoun LimJiyoung JangSeungjae KimTaegyeong KangKangtaek LeeIn-Hong Choi
作者单位: 1Department of Microbiology, Brain Korea 21 Project for Medical Science, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea
2Department of Chemical and Biomolecular Engineering, Yonsei University College of Engineering, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea
刊名: Biomaterials, 2012, Vol.33 (18), pp.4690-4699
来源数据库: Elsevier Journal
DOI: 10.1016/j.biomaterials.2012.03.006
关键词: NanoparticleSilverMacrophageInterleukinmRNA
英文摘要: Abstract(#br)Because of the limited information on size-dependent particle-mediated effects, the present study was conducted to determine if the changes in induced protein expression between 5 nm silver nanoparticles and 100 nm particles after exposure to sub-lethal concentrations. A total of 28,000 cDNA profiles were screened using 5 nm silver nanoparticles and 100 nm silver nanoparticles in a macrophage cell line. Based on results obtained from cDNA microarray we also assessed protein levels of hemeoxygenase-1 (HO-1), heat shock protein-70 (HSP-70) and interleukin-8 (IL-8), which were shown to significantly increase. Together with results obtained using N-acetylcystein (NAC), we were able to clearly show that low level and early stage exposure to 5 nm silver nanoparticles, but not 100...
全文获取路径: Elsevier  (合作)
影响因子:7.604 (2012)

  • macrophages 巨噬细胞
  • inflammatory 激怒的
  • lethal 致死
  • concentrations 浓聚物
  • silver 
  • stress 应力
  • analysis 分析
  • human 人的
  • cDNA 互补 DNA
  • effects 海员自身物品