Lactobacillus reuteri NAD(P)H oxidase: Properties and coexpression with propanediol-utilization enzymes for enhancing 3-hydroxypropionic acid production from 3-hydroxypropionaldehyde
作者: Tarek DishishaRamin Sabet-AzadVictor ArietaRajni Hatti-Kaul
作者单位: 1Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden
2Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, 62511, Beni-Suef, Egypt
刊名: Journal of Biotechnology, 2018
来源数据库: Elsevier Journal
DOI: 10.1016/j.jbiotec.2018.11.010
关键词: NADH oxidaseCo-factor regenerationLactobacillus reuteri3-Hydroxypropionic acid3-HydroxypropionaldehydeFed-batch biotransformation
原始语种摘要: Abstract(#br)Lactobacillus reuteri metabolizes glycerol through propanediol-utilization (Pdu) pathway to 1,3-propanediol (1,3-PD) via 3-hydroxypropionaldehyde (3-HPA) as intermediate. In the resting cells, the oxidized co-factor obtained in the reaction is regenerated by simultaneous oxidation of 3-HPA to 3-hydroxypropionic acid (3-HP) using propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL) and propionate kinase (PduW). We have earlier shown that the use of resting cells of recombinant Escherichia coli expressing the oxidative pathway gives the highest theoretical yield of 1 mol 3-HP per mol 3-HPA but is limited by cofactor depletion. In the present study, the gene encoding the enzyme NAD(P)H oxidase (LreuNox) that utilizes molecular oxygen as substrate, was isolated from...
全文获取路径: Elsevier  (合作)
影响因子:3.183 (2012)

  • propanediol 丙二醇
  • cofactor 辅助因子
  • expression 表示
  • utilization 利用
  • Escherichia 埃希氏菌属
  • regeneration 再生
  • batch 一批
  • recombinant 重组体
  • expressing 压榨
  • highest 最高的