Carbon-13 nuclear magnetic resonance analysis of formaldehyde free preservatives
作者: Kausik DasJoseph DumaisSara BasiagaGary D. Krzyzanowski
作者单位: 1Streck, Inc., 7002 S 109 Street, La Vista, NE 68128, USA
2Agilent Technologies, LSG-Research Products Division-NMR, 5301 Stevens Creek Blvd, Santa Clara, CA 95051, USA
3Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA
刊名: Acta Histochemica, 2013, Vol.115 (5), pp.481-486
来源数据库: Elsevier Journal
DOI: 10.1016/j.acthis.2012.11.004
关键词: Cell-free-DNACell preservativesFormaldehyde-free preservativesFormaldehyde NMRQuantitative 13 C NMR
原始语种摘要: Abstract(#br)Preservation of biomolecules is pivotal in increasingly important molecular diagnostics. Traditionally, formaldehyde is employed for such biomolecular preservation in spite of its carcinogenicity. Moreover, formaldehyde induced cross-linking during fixation is reported to alter structural and functional properties of the preserved biomolecules. Therefore, formaldehyde-free preservatives are advantageous because they are safer for laboratory personnel and they protect the structural and functional integrity of the biomolecules. Streck Cell Preservative and Cell-Free DNA BCT reagents are used as formaldehyde alternative preservatives. However, no studies have been carried out to evaluate formaldehyde concentrations in these preservatives. In this study, we evaluated the free...
全文获取路径: Elsevier  (合作)
影响因子:1.608 (2012)

  • formaldehyde 甲醛
  • preservatives 保存剂
  • resonance 共振
  • analysis 分析
  • laboratory 实验室
  • NMR N-Modular Redundancy
  • preserved 保藏的
  • magnetic 磁的
  • integrity 综合性
  • spectroscopic 光谱的