Development of an intravenous microdialysis method for pharmacokinetic investigations in humans
作者: Jan-Peer ElshoffStephanie Läer
作者单位: 1Institut für Experimentelle und Klinische Pharmakologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
2Klinische Pharmazie und Pharmakotherapie, Universität Düsseldorf, Universitätsstrasse 1, 40225 Düsseldorf, Germany
刊名: Journal of Pharmacological and Toxicological Methods, 2005, Vol.52 (2), pp.251-259
来源数据库: Elsevier Journal
DOI: 10.1016/j.vascn.2005.01.001
关键词: MethodsIn vitro microdialysis cellIntravenous microdialysisRetrodialysis by calibratorPharmacokineticsChildrenSotalol
原始语种摘要: Abstract(#br)Introduction: Limited blood volume is a major problem in pharmacokinetic investigations in specific populations, e.g. children. Intravenous microdialysis might help to obtain improved data sets as it is already successfully done in small animals. Since quantification of drugs is crucial in microdialysis, we developed an in vitro method to produce a workable intravenous microdialysis for human use. Methods: A specifically designed microdialysis cell consisting of glass was heated to 37 °C. The cell was filled with Ringer's solution, plasma or whole blood. A microdialysis probe was inserted into the cell and perfused with Ringer's solution with addition of 4% dextran. The β-receptor blocker sotalol served as a test drug. The stepwise in vitro evaluation process addressed issues...
全文获取路径: Elsevier  (合作)

  • microdialysis 微量渗析
  • sotalol 苏特罗
  • volunteer 志愿兵
  • atenolol 阿替洛尔
  • dialysate 透析液
  • calibrator 校准器
  • plasma 深绿玉髓
  • intravenous 静脉内的
  • blocker 间苗器
  • dextran 葡萄聚糖