Production of 3-hydroxypropionic acid from 3-hydroxypropionaldehyde by recombinant Escherichia coli co-expressing Lactobacillus reuteri propanediol utilization enzymes
作者: Ramin Sabet-AzadRoya R.R. SardariJavier A. Linares-PasténRajni Hatti-Kaul
作者单位: 1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
刊名: Bioresource Technology, 2015, Vol.180 , pp.214-221
来源数据库: Elsevier Journal
DOI: 10.1016/j.biortech.2014.12.109
关键词: Propanediol utilization pathwayLactobacillus reuteri3-Hydroxypropionic acidTwo-step biotransformationRecombinant Escherichia coli
英文摘要: Abstract(#br)3-Hydroxypropionic acid (3-HP) is an important platform chemical for the biobased chemical industry. Lactobacillus reuteri produces 3-HP from glycerol via 3-hydroxypropionaldehyde (3-HPA) through a CoA-dependent propanediol utilization (Pdu) pathway. This study was performed to verify and evaluate the pathway comprising propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL), and propionate kinase (PduW) for formation of 3-HP from 3-HPA. The pathway was confirmed using recombinant Escherichia coli co-expressing PduP, PduL and PduW of L. reuteri DSM 20016 and mutants lacking expression of either enzyme. Growing and resting cells of the recombinant strain produced 3-HP with a yield of 0.3mol/mol and 1mol/mol, respectively, from...
全文获取路径: Elsevier  (合作)
影响因子:4.75 (2012)

  • Escherichia 埃希氏菌属
  • enzymes 酶类
  • recombinant 重组体
  • expressing 压榨
  • utilization 利用
  • pathway 轨道
  • propanediol 丙二醇