Analysis of cholyglycine acid as a biomarker for the early diagnosis of liver disease by fluorescence polarization immunoassay
作者: Ding ShenJie ZhengXiping CuiYingshan ChenQiyi HeRui LvZhaoxia LiSuqing Zhao
作者单位: 1Department of Pharmaceutical Engineering, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China
2Department of Clinical Laboratory, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, PR China
刊名: Sensors & Actuators: B. Chemical, 2018, Vol.256 , pp.846-852
来源数据库: Elsevier Journal
DOI: 10.1016/j.snb.2017.10.013
关键词: CG CholyglycineFPIA fluorescence polarization immunoassayEMIT enzyme multiplied immunoassay techniqueHCC hepatocellular carcinomaEDF fluoresceinthiocarbamyl ethylenediamineFP fluorescence polarizationLOD limit of detectionCR cross-reactivityIQR interquartile rangesCHBV
原始语种摘要: Abstract(#br)Trace concentrations of cholyglycine acid (CG) are useful to reflect the damage degree in liver cells, and useful in the diagnosis and prognosis of liver diseases. Therefore, developing an effective strategy to detect the concentration of CG is of great importance. A homogeneous fluorescence polarization immunoassay (FPIA) was developed for determination of CG concentrations. The effects of tracer concentration, antibody dilution and incubation time on FPIA performance were studied. The FPIA was used to detect CG in sera from 522 patients with liver diseases and 449 healthy controls. The results were compared with those obtained through enzyme multiplied immunoassay technique (EMIT). The FPIA showed a detection limit of 50.9ng/mL for CG and the...
全文获取路径: Elsevier  (合作)
影响因子:3.535 (2012)

  • immunoassay 免疫测定法
  • liver 
  • cholyglycine 甘氨胆酸
  • polarization 极化
  • FPIA 荧光偏振免疫分析
  • fluorescence 荧光
  • keyword 关键词
  • useful 有用的
  • controls 控制机构
  • hepatocellular 肝细胞性的