Production of d -ribose by metabolically engineered Escherichia coli
作者: Hae-Chul ParkYun-Jung KimChang-Wan LeeYong-Taek RhoJeongWoo KangDae-Hee LeeYeong-Je SeongYong-Cheol ParkDaesang LeeSung-Gun Kim
作者单位: 1Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gyeongbuk 39660, Republic of Korea
2Department of Biomedical Science, Youngdong University, Chungbuk 29131, Republic of Korea
3Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea
4Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea
5The 5th R&D Institue-3, Agency for Defense Development, Daejeon 34188, Republic of Korea
刊名: Process Biochemistry, 2017, Vol.52 , pp.73-77
来源数据库: Elsevier Journal
DOI: 10.1016/j.procbio.2016.10.001
关键词: d -RiboseXyloseEscherichia coliTransketolaseCatabolite repression
原始语种摘要: Abstract(#br)Escherichia coli was metabolically engineered for the production of d -ribose, a functional five-carbon sugar, from xylose. For the accumulation of d -ribose, two genes of transketolase catalyzing the conversion of d -ribose-5-phosphate to sedoheptulose-7-phosphate in pentose phosphate pathway were disrupted to create a transketolase-deficient E. coli SGK013. In batch fermentation, E. coli SGK013 grew by utilizing glucose and then started to produce d -ribose from xylose after glucose depletion. E. coli SGK013 produced 0.75g/L of d -ribose, which was identical to the standard d -ribose as confirmed by HPLC and LC/MS analyses. To improve D-ribose production, the ptsG gene encoding the glucose-specific IICB component was disrupted additionally, resulting in...
全文获取路径: Elsevier  (合作)
影响因子:2.414 (2012)

  • Escherichia 埃希氏菌属
  • ribose 核糖
  • engineered 设计的
  • disrupted 断线状
  • xylose 木糖
  • catabolite 降解产物
  • transketolase 酮糖移转酶
  • phosphate 磷酸盐
  • pentose 戊糖
  • functional 功能的