Development of a simple, rapid and high-throughput fluorescence polarization immunoassay for glycocholic acid in human urine
作者: Qiyi HeXiping CuiDing ShenYingshan ChenZhengyun JiangRui LvSergei A. EreminSuqing Zhao
作者单位: 1Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, People’s Republic of China
2Faculty of Chemistry, M. V. Lomonosov Moscow State University, 119991, Moscow, Russia
3National Research Technical University MISiS, 119049, Moscow, Russia
刊名: Journal of Pharmaceutical and Biomedical Analysis, 2018, Vol.158 , pp.431-437
来源数据库: Elsevier Journal
DOI: 10.1016/j.jpba.2018.06.023
关键词: Glycocholic acidFluorescence polarization immunoassayHigh-throughput screeningPolyclonal antibodyUrine
原始语种摘要: Abstract(#br)In this paper, a simple, rapid and high-throughput fluorescence polarization immunoassay (FPIA) based on polyclonal antibodies (PAb) is described for the determination of glycocholic acid (GCA) in human urine. Three fluorescein-labeled GCA (tracers) with different structures and spacer bridges were synthesized and purified by thin-layer chromatography (TLC). The structure effect of tracers on the assay was investigated and the sensitivity of best tracer in the optimized FPIA demonstrated an IC 50 value of 306 ng/mL. The working range of FPIA was 36 ∼ 2 600 ng/mL and the limit of detection (LOD) was 9 ng/mL. The developed FPIA was time-saving that could be completed within 10 min. Human urine samples spiked with GCA were analyzed by this method, followed by confirmation with...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • immunoassay 免疫测定法
  • polarization 极化
  • FPIA 荧光偏振免疫分析
  • urine 尿
  • human 人的
  • throughput 吞吐量
  • screening 筛分
  • fluorescence 荧光
  • GCA Ground Control Approach
  • rapid 急流急滩