Purification, characterization and gene identification of a membrane-bound glucose dehydrogenase from 2-keto- d -gluconic acid industrial producing strain Pseudomonas plecoglossicida JUIM01
作者: Da-Ming WangLei SunWen-Jing SunFeng-Jie CuiJin-Song GongXiao-Mei ZhangJin-Song ShiZheng-Hong Xu
作者单位: 1The Key Laboratory of Industrial Biotechnology, Ministry of Education, National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China
2School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China
3Parchn Sodium Isovitamin C Co., Ltd., Dexing 334221, PR China
刊名: International Journal of Biological Macromolecules, 2018, Vol.118 , pp.534-541
来源数据库: Elsevier Journal
DOI: 10.1016/j.ijbiomac.2018.06.097
关键词: Pseudomonas plecoglossicida2-Keto- d -gluconic acid (2KGA)Glucose dehydrogenasePurificationGene identification
原始语种摘要: Abstract(#br)The membrane-bound glucose dehydrogenase (mGDH) is a rate-limiting enzyme for the industrial production of 2-keto- d -gluconic acid (2KGA) from glucose. In this study, mGDH was firstly purified from a 2KGA industrial producing strain Pseudomonas plecoglossicida JUIM01. The purified mGDH exhibited a specific activity of 16.85 U/mg and was identified as monomeric membrane-bound PQQ-dependent dehydrogenase with a molecular mass of ~87 kDa. The K m and V max value of d -glucose were 0.042 mM and 14.620 μM/min, and the optimal pH and temperature were of 6.0 and 35 °C with favorable acid resistance and poor heat tolerance. Ca 2+ /Mg 2+ showed a significantly positive effect on mGDH activity with 20% increase, whereas EDTA/EGTA had a negative influence, and Ca 2+ was essential for...
全文获取路径: Elsevier  (合作)

  • identification 辨认
  • producing 产油
  • glucose 葡萄糖
  • strain 应变
  • dehydrogenase 脱氢酶
  • membrane 
  • bound 
  • industrial 工业的