Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains
作者: Kathrin TomasekTobias BergmillerCălin C. Guet
作者单位: 1Institute of Science and Technology (IST) Austria, Klosterneuburg, Austria
刊名: Journal of Biotechnology, 2018, Vol.268 , pp.40-52
来源数据库: Elsevier Journal
DOI: 10.1016/j.jbiotec.2018.01.008
关键词: Flow cytometryGene expressionCell viabilityEscherichia coliOuter membrane
原始语种摘要: Abstract(#br)Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals. Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts. These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability. We observed buffer-related effects in several different E. coli strains, K-12, C and W, but not E. coli B, which can be partially explained by differences in...
全文获取路径: Elsevier  (合作)
影响因子:3.183 (2012)

  • Escherichia 埃希氏菌属
  • membrane 
  • viability 生活力
  • fluorescence 荧光
  • expression 表示
  • dilution 稀度
  • bacterial 细菌的
  • fluorescent 萤光的
  • cytometry 血细胞计数
  • stability 稳定性