Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes
作者: Zhen ChenHongjuan LiuDehua Liu
作者单位: 1Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China
2Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, PR China
刊名: Enzyme and Microbial Technology, 2009, Vol.45 (4), pp.305-309
来源数据库: Elsevier Journal
DOI: 10.1016/j.enzmictec.2009.04.005
关键词: dhaTdhaD3-Hydroxypropionaldehyde13-PropanediolKlebsiella pneumoniae
原始语种摘要: Abstract(#br)3-Hydroxypropionaldehyde (3-HPA), an important intermediary metabolite of 1,3-propanediol (PDO) production, would be toxic to the cell growth and led to the abnormal cessation of the fermentation process. In this study, the dhaD gene encoding glycerol dehydrogenase (GDH) and dhaT gene encoding 1,3-propanediol oxidoreductase (PDOR) were overexpressed in Klebsiella pneumoniae ACCC 10082 to decrease the 3-HPA accumulation and increase the coenzyme NADH supply. By the construction of pTD plasmid, GDH and PDOR were both overexpressed and their enzyme activities were increased by 2.6- and 3.2-fold, respectively. The enzyme activity ratio of PDOR/GDHt (glycerol dehydratase) also was increased. On the other hand, NADH production was enhanced and the ratio of NADH/NAD + exceeded 1...
全文获取路径: Elsevier  (合作)
影响因子:2.592 (2012)

  • accumulation 堆积
  • dehydratase 脱水酶
  • oxidoreductase 氧化还原酶
  • inducement 诱因
  • PDO Power Door Opening
  • increased 增加
  • propanediol 丙二醇
  • batch 一批
  • strain 应变
  • glycerol 甘油