VPAC 1 receptors have different agonist efficacy profiles on membrane and intact cells
作者: Ingrid LangerPascale VertongenJason PerretJohnny CnuddeFrançoise GregoirePhilippe De NeefPatrick RobberechtMagali Waelbroeck
作者单位: 1Department of Biological Chemistry and Nutrition, Faculty of Medicine, Université Libre de Bruxelles, Bat GE, CP 611, 808 Route de Lennik, B-1070 Brussels, Belgium
刊名: Cellular Signalling, 2002, Vol.14 (8), pp.689-694
来源数据库: Elsevier Journal
DOI: 10.1016/S0898-6568(02)00009-8
关键词: VPAC 1 receptorsVIPVIP analogsAdenylate cyclase[Ca 2+ ] i
原始语种摘要: Abstract(#br)The vasoactive intestinal peptide receptor VPAC 1 is preferentially coupled to G αs protein but also increases [Ca 2+ ] i through interaction with G αi /G αq protein. We evaluated a panel of full, partial and null agonists for their capability to stimulate adenylate cyclase activity in both intact cells and membrane and [Ca 2+ ] i in intact cells transfected with the reporter gene aequorin. In intact cells, the agonists efficacy for cAMP and calcium increase were well, but not linearly correlated: VPAC 1 receptors activated G αs protein more efficiently but with the same pharmacological profile as the other G proteins. In contrast, there was a difference between cAMP increase in intact and broken cell membranes: EC 50 values were generally lower in intact cells whereas the...
全文获取路径: Elsevier  (合作)
影响因子:4.304 (2012)

  • intact 完整的
  • cells 麻风细胞
  • efficacy 效验
  • membrane 
  • protein 蛋白质
  • different 不相同的
  • VIP Variable Information Processing
  • agonist 织肌
  • analogs 类似物
  • cAMP 环腺苷酸