Hyperosmotic Stress Induces Cell Death in an Odontoblast-lineage Cell Line
作者: Mari FujisawaMasayuki TokudaYoko Morimoto-YamashitaShoko TatsuyamaSzilvia AranyToshihiro SugiyamaChiaki KitamuraYoshiyuki ShibukawaMitsuo Torii
作者单位: 1Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
2Department of Biochemistry, Akita University Graduate School of Medicine, Akita, Japan
3Department of Cariology and Periodontology, Division of Pulp Biology, Operative Dentistry and Endodontics, Kyushu Dental College, Kitakyushu, Japan
4Department of Physiology, Tokyo Dental College, Chiba, Japan
刊名: Journal of Endodontics, 2012, Vol.38 (7), pp.931-935
来源数据库: Elsevier Journal
DOI: 10.1016/j.joen.2012.03.023
关键词: Cell deathhyperosmotic stressMAPKodontoblast cellssucrose
英文摘要: Abstract(#br)Introduction(#br)Osmotic stress is one of the stimulations related to dental pain caused by caries or dentin hypersensitivity. The mechanism of osmotic-induced dental pain is not completely understood. The purpose of this study was to examine the responses of odontoblasts under sucrose-induced hyperosmotic stress.(#br)Methods(#br)We used an odontoblast-lineage cell (OLC) line in our experiments. OLCs were stimulated with sucrose to produce hyperosmotic stress. The expressions of dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP 1) were detected by using reverse transcriptase polymerase chain reaction assay. The cell viability of OLCs was detected by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium assay. The responses accompanied with cell...
全文获取路径: Elsevier  (合作)
影响因子:2.929 (2012)

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