Errors in measuring plasma free fatty acid concentrations with a popular enzymatic colorimetric kit
作者: Yilin SongLianzhen ZhouMichael D. Jensen
作者单位: 1Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China
2Division of Endocrinology, Diabetes and Metabolism, Endocrine Research Unit, Mayo Clinic, 200 1st Street SW, Rm 5-194 Joseph, Rochester, MN 55905, USA
刊名: Clinical Biochemistry, 2019, Vol.66 , pp.83-90
来源数据库: Elsevier Journal
DOI: 10.1016/j.clinbiochem.2019.01.008
关键词: Liquid chromatographyMass spectrometryDiagnostic toolsFatty acidLipidsMethods/HPLCOleate
英文摘要: Abstract(#br)Objectives(#br)Our goal was to test whether an enzymatic, colorimetric assay, the WAKO NEFA kit, provides information equivalent to liquid chromatography (LC) LC-based measures of free fatty acid (FFA).(#br)Design & methods(#br)We reanalyzed nadir FFA samples from 109 volunteers from a previous study where we demonstrated that maximal suppression of FFA concentrations predicts metabolic abnormalities in humans; the results from the WAKO NEFA kit, which has been widely used for over three decades, could not replicate our findings. We conducted additional studies to directly compare results from this kit to our LC-mass spectrometry (LC/MS) method that was validated by our LC-UV detection method.(#br)Results(#br)Plasma samples with FFA concentrations ranging from 0.015 to 1.813...
全文获取路径: Elsevier  (合作)
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来源刊物:
影响因子:2.45 (2012)

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关键词翻译
关键词翻译
  • enzymatic 酶催的
  • colorimetric 比色的
  • concentrations 浓聚物
  • plasma 深绿玉髓
  • fatty 脂肪的
  • spectrometry 光谱测定
  • popular 普及
  • measuring 测量
  • chromatography 色层分离法
  • tools 工具