Eucommia ulmoides flavones ( EU F) abrogated enterocyte damage induced by LPS involved in NF-κB signaling pathway
作者: Tarique HussainDiaxiu YuanBie TanGhulam MurtazaNajma RahuMuhammed Saleem KalhoroDildar Hussain KalhoroYulong Yin
作者单位: 1Laboratory of Animal Nutritional Physiology and Metabolic Processes, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan, China
2University of the Chinese Academy of Sciences, Beijing 10008, China
3Animal Sciences Division, Nuclear Institute for Agriculture and Biology (NIAB), P.O.Box: 128, Jhang Road, Faisalabad, Pakistan
4Department of Medicine, Jishou University, Jishou, Hunan 416000, China
5Shaheed Benazir Bhutto University of Veterinary & Animal Sciences, Sakrand, Sindh 67210, Pakistan
6Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Sindh 70050, Pakistan
刊名: Toxicology in Vitro, 2020, Vol.62
来源数据库: Elsevier Journal
DOI: 10.1016/j.tiv.2019.104674
关键词: Eucommia ulmoides flavonesInflammationPI3K-NFκB signaling pathwayEnterocytes and LPS
英文摘要: Abstract(#br)This study was conducted to explore the regulatory mechanism of Eucommia ulmoides flavones ( EU F) using enterocyte damage model induced by lipopolysaccharide (LPS). Intestinal porcine epithelial cell line (IPEC-J2) cells were cultured in Dulbecco's modified eagle medium with high glucose (DMEM-H) medium containing 0 or 10 μg/mL EU F, 0 or 40 ng/mL LPS. The results showed that LPS impaired DNA synthesis, cell viability, mitochondrial function, arrested cell cycle and induced apoptosis, reduced SOD activity while the EU F treated cells provided beneficial effect on all these parameters (P < 0.05). The addition of EU F increased phosphorylated Akt, IκBα and phosphorylated IKKα/β, but decreased Bax and Caspase-3 protein expressions in LPS-treated cells (P < 0.05). For the second...
全文获取路径: Elsevier  (合作)
影响因子:2.65 (2012)

  • LPS Laboratory Peripheral System
  • pathway 轨道
  • signaling 发信号
  • enterocyte 肠上皮细胞
  • involved 涉及
  • induced 感应的
  • damage 损伤