Mechanism of Phage PS166-Mediated Biotype Conversion in Vibrio cholerae: Role of the hly A Locus
作者: Rupa MukhopadhyayRanajit K. Ghosh
作者单位: 1Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, Calcutta, 700032, India
刊名: Virology, 2000, Vol.273 (1), pp.44-51
来源数据库: Elsevier Journal
DOI: 10.1006/viro.2000.0359
原始语种摘要: Abstract(#br)Temperate phage PS166 lysogens of Vibrio eltor MAK757 biotype eltor belong to two major categories. Seventy percent of the lysogens acquire auxotrophy for glycine and histidine and maintain their parental biotype. About 10% of the lysogens become Cys − or Cys − Met − and are converted to the classical biotype with complete changes in all biotype-specific determinants. PCR and RFLP analysis revealed that in the latter lysogens, the phage genome integrated at the hly A locus, whereas the same locus remained unaffected in lysogens that retained their parental biotype. These results suggest that the two types of lysogens arose due to integration of the phage genome at two different locations on the chromosome. A restriction map of the phage genome was constructed using Ava II and...
全文获取路径: Elsevier  (合作)
影响因子:3.367 (2012)

  • biotype 生物型
  • locus 轨迹
  • phage 噬菌体
  • distinguish 辨别
  • genome 基因组
  • whereas 鉴于
  • parental 父母
  • carrying 垫纱
  • auxotrophy 营养缺陷型
  • unaffected 末触动的