DNA/chitosan electrostatic complex
作者: Lourdes Mónica Bravo-AnayaJ.F. Armando SolteroMarguerite Rinaudo
作者单位: 1Universidad de Guadalajara, Departamento de Ingeniería Química, Blvd. M. García Barragán #1451, C.P. 44430 Guadalajara, Jalisco, Mexico
2Grenoble Alpes University, LRP, F-38000 Grenoble, France
3Biomaterials Applications, 6 Rue Lesdiguières, 38000 Grenoble, France
刊名: International Journal of Biological Macromolecules, 2016, Vol.88 , pp.345-353
来源数据库: Elsevier Journal
DOI: 10.1016/j.ijbiomac.2016.03.035
关键词: DNAChitosanElectrostatic interactionsCompactionStability
原始语种摘要: Abstract(#br)Up to now, chitosan and DNA have been investigated for gene delivery due to chitosan advantages. It is recognized that chitosan is a biocompatible and biodegradable non-viral vector that does not produce immunological reactions, contrary to viral vectors. Chitosan has also been used and studied for its ability to protect DNA against nuclease degradation and to transfect DNA into several kinds of cells. In this work, high molecular weight DNA is compacted with chitosan. DNA-chitosan complex stoichiometry, net charge, dimensions, conformation and thermal stability are determined and discussed. The influence of external salt and chitosan molecular weight on the stoichiometry is also discussed. The isoelectric point of the complexes was found to be directly related to the...
全文获取路径: Elsevier  (合作)

  • chitosan 壳聚糖
  • DNA Deoxyribonucleic Acid
  • complex 超群
  • biocompatible 生物相容的
  • biodegradable 生物降解的
  • neutralization 中和
  • phosphate 磷酸盐
  • immunological 免疫学的
  • isoelectric 等电离的
  • nuclease 核酸酶