Identification and functional analyses of polymorphism haplotypes of protein phosphatase 2A-Aα gene promoter
作者: Yu-Chun LinHui-Feng ChenLi-Na LinJie LuoWen LiShu-Jiang ZhangXiao-Jie LiYao-Ming HuYu-Xi ChenWen ChenZhong-Ning Lin
作者单位: 1Faculty of Preventive Medicine, Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou, PR China
刊名: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2011, Vol.716 (1), pp.66-75
来源数据库: Elsevier Journal
DOI: 10.1016/j.mrfmmm.2011.08.004
关键词: Protein phosphatase 2AAα subunitPPP2R1A gene promoterPolymorphismHaplotypeFunctional analysis
原始语种摘要: Abstract(#br)The serine–threonine protein phosphatase 2A (PP2A) is a heterotrimeric enzyme family that plays an essential regulatory role in cell growth, differentiation, and apoptosis. Mutations in the genes encoding PP2A-Aα/β subunits are associated with tumorigenesis and other human diseases. To explore whether genetic variations in the promoter region of the PP2A-Aα gene ( PPP2R1A ) and their frequent haplotypes in the Han Chinese population have an impact on transcriptional activity, we collected DNA samples from 63 healthy Chinese donors and searched for genetic variations in the 5′-flanking promoter region of PPP2R1A ( PPP2R1Ap ). Haplotypes were characterized by Haploview analysis and individual subcloning. A set of molecular and functional experiments was performed using reporter...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • promoter 催化剂
  • functional 功能的
  • genetic 遗传的
  • subcloning 亚克隆
  • region 地域
  • phosphatase 磷酸酶
  • protein 蛋白质
  • polymorphism 多晶形
  • PPP Parallel Pattern Processor
  • luciferase 荧光素酶