Insufficient PINX1 expression stimulates telomerase activation by direct inhibition of EBV LMP1-NF-κB axis during nasopharyngeal carcinoma development
作者: Yunyi LiuPinggui GongNi ZhouJunjun ZhangCui HeShuilian WangHong Peng
作者单位: 1Guangdong Provincial Second People's Hospital, Southern Medical University, Guangzhou, 510317, Guangdong, China
2The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510282, Guangdong, China
刊名: Biochemical and Biophysical Research Communications, 2019, Vol.514 (1), pp.127-133
来源数据库: Elsevier Journal
DOI: 10.1016/j.bbrc.2019.04.104
关键词: PINX1NF-κBTelomeraseNasopharyngeal carcinomaEpstein-Barr virus
英文摘要: Abstract(#br)Objective(#br)Early malignant transformation of nasopharyngeal carcinoma(NPC) is associated with Epstein-Barr virus(EBV) infection and telomerase activation. The EBV latent membrane protein 1(LMP1) regulates expression of various genes by triggering NF-κB signaling pathway. PINX1 is a well-identified tumor suppressor gene by inhibiting telomerase activity and cancer cell growth. However, whether and how EBV inhibit PINX1 expression and activate telomerase in NPC is still incompletely elucidated.(#br)Methods(#br)Immunohistochemistry, real-time PCR and Western blotting were utilized to explore the expression of PINX1. Chromatin immunoprecipitation(ChIP) and Dual-luciferase reporter assay were used to elucidate the regulatory mechanism between NF-κB and PINX1. TRAP-SYBR Green...
全文获取路径: Elsevier  (合作)

  • nasopharyngeal 鼻咽的
  • EBV Extended Binary Vectors
  • carcinoma 癌症
  • inhibition 抑制
  • telomerase 未端酶
  • development 开发
  • expression 表示
  • activation 活化
  • direct 直接的
  • during 在的期间