Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation
作者: Robert GlydeFuzhou YeMilija JovanovicIoly Kotta-LoizouMartin BuckXiaodong Zhang
作者单位: 1Section of Structural Biology, Department of Medicine, Imperial College London, London SW7 2AZ, UK
2Department of Life Sciences, Imperial College London, London SW7 2AZ, UK
刊名: Molecular Cell, 2018, Vol.70 (6), pp.1111-1120.e3
来源数据库: Elsevier Journal
DOI: 10.1016/j.molcel.2018.05.021
关键词: Transcription initiationDNA openingTranscription bubbleRNA polymeraseSigmaDe novo RNA synthesisProtein-DNA interactionsPromoter DNATranscription activationBacterial enhancer-binding proteins
原始语种摘要: Summary(#br)Gene transcription is carried out by multi-subunit RNA polymerases (RNAPs). Transcription initiation is a dynamic multi-step process that involves the opening of the double-stranded DNA to form a transcription bubble and delivery of the template strand deep into the RNAP for RNA synthesis. Applying cryoelectron microscopy to a unique transcription system using σ 54 (σ N ), the major bacterial variant sigma factor, we capture a new intermediate state at 4.1 Å where promoter DNA is caught at the entrance of the RNAP cleft. Combining with new structures of the open promoter complex and an initial de novo transcribing complex at 3.4 and 3.7 Å, respectively, our studies reveal the dynamics of DNA loading and mechanism of transcription bubble stabilization that involves coordinated,...
全文获取路径: Elsevier  (合作)
影响因子:15.28 (2012)

  • transcription 地名转写
  • strand 海滨
  • DNA Deoxyribonucleic Acid
  • promoter 催化剂
  • subunit 亚单位
  • stabilization 稳定
  • initiation 创始
  • complex 超群
  • universally 普遍地