Our experience regarding withdraw blood from rats and preparation of platelet-rich plasma
作者: Ali Gokkaya Metin Gorgu Jehat Kizilkan Ertugrul Karanfil Ali Dogan
刊名: Experimental Biomedical Research, 2019, Vol.2 (1)
来源数据库: Experimental Biomedical Research Editorial Office
DOI: 10.30714/j-ebr.2019147580
关键词: RatBlood withdraw techniquesPlatelet rich plasma
原始语种摘要: Aim: Withdraw blood from rats is an important, but not easy, invasive procedure in experimental research on these animals. In addition, the preparation and standardization of platelet-rich plasma(PRP) is a more difficult process in rats. In this study, we presented our experiences about rat blood collection and PRP preparation technique. Methods and Result: This experimental study was performed with ten male Wistar rats weighing 250–300 g. Under anesthesia, the blood was obtained by percutaneous puncture from the right ventricle of the rats.The blood obtained from rats was rapidly transferred to tubes containing anticoagulants such as sodium citrate or acid citrate dextrose solution A. After the first centrifuge, the all plasma was collected by a pipette after a second spin in a sterile...
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  • withdraw 夺取
  • plasma 深绿玉髓
  • preparation 预备
  • blood 血液
  • Blood 
  • collection 收集
  • procedure 手续
  • concentration 浓度
  • anesthesia 感觉缺失
  • sterile 无生殖力的