Background: Motivation is crucial for organizations to function; without motivation the organization would be less efficient. Objective: To assess the role of motivation on technicians and teaching assistants performance in College of Medical Technology in Derna, city. Methodology: A cross- sectional design was used to conduct the study. The target population of the study was technicians and teaching assistants working in College of Medical Technology. Data was collected using a questionnaire and analyzed by percentage, mean, range, rank, frequency and standard deviation. Results: Among 39 study sample 11 were male and 28 were female. Most of them were within age group of 21- 30 years (87.18%). The study reveals that, “training” was ranked first as the most important motivational factor,... followed by second rank was shared between “salary and job security”. Few of the study sample received incentives in form of financial incentive, clothing allowance, phone and petrol cards. And said the incentive has a positive impact on job performance. All participants said would do better job if they were motivated. Conclusion: The findings of this study indicate that motivational factors such as “training, salary, and job security” are major motivational factors, which can lead to better services delivery in the college as it brings positive results on the technicians and teaching assistant’s performance. The study also revealed that incentives available to technicians and teaching assistants in the college are inadequate.