AFLP Analysis on Genetic Diversity of P. polyphylla var. yunnanensis
滇重楼遗传多样性的AFLP分析
作者: 李艳冰王玲何忠俊谢世清廖秋红杨生超刘涛
作者单位: 1云南农业大学农学与生物技术学院,云南,昆明
2西南中药材种质创新与利用国家地方联合工程研究中心,云南,昆明
3云南省优势中药材规范化种植工程研究中心,云南,昆明
刊名: Botanical Research, 2018, Vol.07 (01), pp.45-53
中文刊名: 植物学研究, 2018, Vol.07 (01), pp.45-53
来源数据库: Hans Pubs Journal
DOI: 10.12677/BR.2018.71007
关键词: P. polyphylla var. yunnanensis
中文关键词: 滇重楼种质资源遗传多样性AFLP
英文摘要: Objective: To evaluate the genetic diversity of germplasm resources for P. polyphylla var. yunna-nensis and provide biological reference for germplasm resources protection, genetic relationship analysis, breeding, and introduction and cultivation of P. polyphylla var. yunnanensis. Methods: Amplified fragment length polymorphism (AFLP) marker was developed to analyze the genetic diversity among 15 samples of P. polyphylla var. yunnanensis from various habitats in Yunnan re-gion, and cluster analysis and principal coordinate analysis were revealed by the NTSYS software. Results: At the species level, the percentage of polymorphic loci (PPL) was 88.15%. Shannon’s in-formation index (I) was 0.4158. Nei’s genetic diversity index was 0.2533. The total genetic diversity (Ht) was 0.2519, the...
中文摘要: 目的:探索云南滇重楼(Paris polyphylla var. yunnanensis)种质资源的遗传多样性,为滇重楼种质资源保护、亲缘关系研究、品种选育和引种栽培提供分子生物学依据。方法:利用AFLP 标记对来自云南不同产地的15份滇重楼开展遗传多样性分析,采用NTSYS软件进行聚类和主坐标分析。结果:在物种水平上,多态位点百分率(PPL)为48.25%,Shannon信息指数(I)为0.3903,Nei’s基因多样性指数平均为0.2768群体总的遗传多样性(Ht)为0.2519,而居群内的遗传多样性(Hs)为0.1868,群体间遗传分化系数Gst为0.2583,即居群间的遗传变异占总变异的25.84%,而居群内的遗传变异为74.17%。结论:AFLP标记的结果表明滇重楼的遗传多样性程度较低,群体间分化程度很低。居群间基因流(Nm)为0.7178,表明居群之间遗传交换较少。
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