Effect of Zishenpingchan Granule on Neurobehavioral Manifestations and the Activity and Gene Expression of Striatal Dopamine D1 Receptors of Rats with Levodopa-Induced Dyskinesias
滋肾平颤颗粒对异动症大鼠行为学及纹状体多巴胺D1受体活性和基因表达的影响
作者: 叶青高东雯周洁袁晓蕾袁灿兴
作者单位: 1上海中医药大学附属龙华医院脑病科
2上海中医药大学附属龙华医院超声科
刊名: Traditional Chinese Medicine, 2017, Vol.06 (02), pp.118-126
中文刊名: 中医学, 2017, Vol.06 (02), pp.118-126
来源数据库: Hans Pubs Journal
DOI: 10.12677/TCM.2017.62020
关键词: LID Rats
中文关键词: 异动症大鼠,滋肾平颤颗粒,行为学,多巴胺D1受体,直接通路
英文摘要: Objective: To observe the effects of Traditional Chinese Medicine on neurobehavioral manifestations and the activity and mRNA expression of striatal dopamine D1 receptors of rats with levodo-pa-induced dyskinesias (LID). Methods: The rat model of Parkinson’s disease (PD) was established by 6-OHDA; then, the model of LID in rat was produced by injecting levodopa (LD) and benserazide for 4 weeks. The rats were divided into normal control group, LID model group and TCM interven-tion group. After 4 weeks of treatment, the effect of TCM on abnormal involuntary movement (AIM) score of rats with LID, peak dose rotation and efficacy time of levodopa (LD) were observed. The maximum binding capacity (Bmax) and equilibrium dissociation constant (KD) of striatal dopamine D1 receptors of rats were...
中文摘要: 目的:观察中药对异动症(levodopa-induced dyskinesias, LID)大鼠行为学和纹状体多巴胺D1受体活性及mRNA表达的影响。方法:6-OHDA构建(Parkinson’s disease, PD)大鼠模型,左旋多巴治疗4周制作LID大鼠模型,将成功建立的LID大鼠,随机分为模型组、中药干预组,另取相同数量的正常大鼠作为正常对照组,治疗四周后观察中药对LID大鼠异常不自主运动(abnormal involuntary move-ment, AIM)评分、剂峰旋转次数的影响;测定大鼠纹状体多巴胺D1受体的最大结合容量(maximum binding capacity, Bmax)和平衡解离常数(equilibrium dissociation constant, KD);运用实时定量PCR检测多巴胺D1受体mRNA表达。结果:模型组大鼠随着左旋多巴治疗时间的延长,AIM评分及剂峰旋转次数进行性升高;与模型组比较,中药干预后可明显减少异动症大鼠的AIM积分及旋转次数(P < 0.05)。纹状体多巴胺D1受体活性结果显示:两组大鼠与正常对照组比较Bmax升高(P < 0.01),KD值降低(P < 0.05),受体活性明显增高,中药治疗可使Bmax降低,KD值升高(P < 0.01),明显降低D1受体活性;荧光实时定量PCR结果显示模型组大鼠多巴胺D1受体mRNA表达明显上调(P < 0.01);中药可以显著下调多巴胺D1受体mRNA的表达(P < 0.05)。结论:多巴胺D1受体介导的直接通路过度激活可能是异动症产生的主要机制。中药主要是通过下调多巴胺D1受体活性及基因表达从而有效缓解异动症症状。
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