Real-Time Video Imaging of Protease Expression In Vivo
作者: Lei ZhuJin XieMagdalena SwierczewskaFan ZhangQimeng QuanYing MaXuexun FangKwangmeyung KimSeulki LeeXiaoyuan Chen
刊名: Theranostics, 2011, Vol.1 , pp.18-27
来源数据库: Ivyspring Journal
原始语种摘要: We demonstrate the first true real-time in vivo video imaging of extracellular protease expression using an ultrafast-acting and extended-use activatable probe. This simple, one-step technique is capable of boosting fluorescent signals upon target protease cleavage as early as 30 minutes from injection in a small animal model and is able to sustain the strong fluorescent signal up to 24 hours. Using this method, we video imaged the expression and inhibition of matrix metalloproteinases (MMPs) in a tumor-bearing mouse model. The current platform can be universally applied to any target protease of interest with a known peptide substrate and is adaptable to a wide range of real-time imaging applications with high throughputs such as for in vivo drug screening, examinations of the...
全文获取路径: Ivyspring 

  • fluorescent 萤光的
  • expression 表示
  • animal 动物的
  • protease 朊酶
  • target 目标
  • screening 筛分
  • universally 普遍地
  • small 小的
  • mouse 小鼠
  • platform 台地