Interaction Characteristic Studies of Ciprofloxacin and/or Sulphadiazine with Bovine Serum Albumin by Spectroscopic Technique
作者: Xu-Yang Liu Qian Wang Zhi-Hong Shi Xiang-Hua XiaHan-Wen Sun
刊名: Asian Journal of Chemistry, 2015, Vol.27 (3)
来源数据库: Asian Journal of Chemistry
DOI: 10.14233/ajchem.2015.17121
关键词: FluorescenceBovine serum albuminCiprofloxacinSulphadiazineInteraction characteristics.
原始语种摘要: In this paper, the interaction of ciprofloxacin (CPFX) and/or sulphadiazine (SD) with bovine serum albumin (BSA) under simulative physiological conditions was studied, by fluorescence quenching in combination with UV-visible spectroscopic method. The fluorescence quenching constants, binding distance and binding constants for BSA-CPFX and/or sulphadiazine systems were determined. The fluorescence quenching of BSA by addition of ciprofloxacin and/or sulphadiazine is due to static quenching and energy transfer. The binding constants (KA) of BSA-SD and BSA-CPFX systems were 4.04 × 106 and 6.96 × 105 M-1, respectively, showing that sulphadiazine has higher binding capability with bovine serum albumin than ciprofloxacin. In the presence of sulphadiazine or ciprofloxacin, the KA values of...
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  • sulphadiazine 磺胺嘧啶
  • ciprofloxacin 卷须霉素
  • fluorescence 荧光
  • constants 恒有种
  • pharmacology 药理学
  • BSA 二进制同步适配器
  • capability 能力
  • quenching 猝熄
  • conformation 构象
  • biochemistry 生化学