Obesity is a common and serious medical problem that adverse health consequences and associated with many serious disease. FTO gene plays a main role in obesity. Bioinformatics’ analysis of FTO gene initiated by Polyphen-2 and SIFT server was used to review 31pathological polymorphisms. Among these 31, 10 pathological polymorphisms were found to be very damaging, with higher Polyphen-2 score of the Polyphen-2 server (=1) and a SIFT tolerance index of 0.000-0.005. Protein structural analysis was done by modeling of amino acid substitutions using Project Hope server for all these pathological polymorphisms. 30 SNPs in the 3’URT containing 63 alleles can be disrupted by a conserved miRNA site. Hence, we hope our results will provide useful information that needed to help researchers to do... further study to solve the obesity problem in the future. We considered this study a distinctive one, because there is no researches deal with matter in silico studies.