Article: Transfection optimisation in HEK-293 cell line - Asian Journal of Medical and Biological Research
作者: Jay Prakash Sah M. Salahuddin M. Abu Sayed Abu Saim Al Salauddin Md. Sayfullah Mahjabin Rashid Saidin Saclain Mizanur Rahman Washim Muhammad Aurang Zeb Deluwer Hossain Md. Rayhan Chowdhury M. A. Momin
刊名: Asian Journal of Medical and Biological Research, 2015, Vol.1 (1), pp.121-129
来源数据库: Asian Journal of Medical and Biological Research
原始语种摘要: Transfection is the most popular experiments among biological scientist to study the gene expression. But it is really tedious to find out the optimum conditions for protein expression for transfection while working with plasmid DNA. In our present study, the main objective was to setup suitable conditions to study the protein expression. From different conditions, protein expression was observed for 2μg of DNA. We tried different cell number and time frame and got 6.25×105 cell/ well of 6 well and 24 hour incubation were the best condition for maximum expression of protein. In our experiment 1:3 ratio of DNA: Lipfectamine was the best for optimum expression of protein in mammalian cell line.
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  • Article 物品
  • expression 表示
  • protein 蛋白质
  • transfection 转染
  • observed 观察到的
  • conditions 条件式
  • incubation 孵化
  • mammalian 哺乳动物
  • plasmid 质粒
  • objective 接物镜