Effect of dimethyl sulfoxide on in vitro proliferation of skin fibroblast cells
作者: Mahipal SinghKya McKenzieXiaoling Ma
刊名: Journal of Biotech Research, 2017(8)
来源数据库: Bio Tech System
原始语种摘要: Dimethyl sulfoxide is a preferred solvent for a wide range of compounds. It is used as a solvent of drugs for invitro toxicity testing using cell lines. Determination of safe solvent concentration for cell type to be used is a prerequirement. Here we show that 0.1% DMSO in growth media is the safest concentration for skin fibroblast cell line used in this study. The low concentrations (0.01-0.001%) enhance proliferation of cells when cultured in-vitro on plastic surfaces for a 4-day period, whereas higher concentrations (0.5-3%) lead to reduction in cell-viability on a dose dependent manner. No cell survived beyond 3% DMSO.
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  • fibroblast 成纤维细胞
  • vitro 体外
  • concentration 浓度
  • sulfoxide 亚砜
  • preferred 可取
  • whereas 鉴于
  • solvent 溶媒
  • higher 
  • cells 麻风细胞
  • viability 生活力