Characterization of a highly repetitive DNA sequence in Camellia sinensis (L.) O. Kuntze genome
作者: Mahipal SinghBana DhimanChan Sharma
刊名: Journal of Biotech Research, 2011(3)
来源数据库: Bio Tech System
原始语种摘要: Tea is a widely consumed non-alcoholic natural beverage grown mostly in tropical asia and Africa. It is a vegetatively propagated crop and thus has low genetic variability. Repetitive DNA sequences in the genome, although, are commonly recognised very useful to differentiate individuals, they have not been explored extensively in tea genome. Here we report characterization of novel repetitive motiffs in a genomic DNA clone, designated as pMST11. It was isolated and sequenced from a genomic library of Camellia sinensis (L.) O. Kuntze by reverse genomic hybridisation approach. Sequence analysis of the insert displayed a highly A+T rich (66.8%) fragment of 894 base pairs (bp) containing a 168 bp single open reading frame in minus strand. The insert exhibited twelve 15-45 bp long direct...
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关键词翻译
关键词翻译
  • genome 基因组
  • repetitive 重复
  • genomic 染色体组的
  • sequence 次序
  • DNA Deoxyribonucleic Acid
  • genetic 遗传的
  • contained 容纳的
  • insert 插入
  • dinucleotide 二核苷酸
  • consensus 合意