Characterization of GSF289: a fibroblast cell line derived from goat ear skin explants
作者: Mahipal SinghAnil K. SharmaPushpa Yadav
刊名: Journal of Biotech Research, 2011(3)
来源数据库: Bio Tech System
原始语种摘要: Recently we established three fibroblast cell lines from ear skin explants of normal healthy dairy goats, of Kiko and Saanen breed (In Vitro Cell.Dev.Biol.-Animal; doi: 10.1007/s11626-010-9373-4). The cell lines revealed a viability rate of 96.2%, displayed a typical ‘S’ shaped growth curve with a population doubling time of 25 hours, and were free from microbial, fungal and mycoplasma contamination. We further characterized these cell lines and, in this communication we show the cytogenetic analysis and the genetic transfection of GSF289 cells. The GSF289 cell line which originated from Saanen breed of goats was successfully transfected with pcDNA3.1/NTGFP plasmid vector containing green fluorescent protein (GFP) gene under human cytomegalovirus (CMV) promoter. The efficiency of...
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关键词翻译
关键词翻译
  • fibroblast 成纤维细胞
  • genetic 遗传的
  • transfection 转染
  • fluorescent 萤光的
  • plasmid 质粒
  • manipulation 操作
  • viability 生活力
  • additional 追加的
  • lines 线型
  • further 进一步