Keerti Soundappan, Richa Bajaj, Brajesh N, a Vaidya, Nirmal Joshee
||Journal of Biotech Research, 2018(9)
||Bio Tech System
Curry leaf (Curry Patta) tree is an economically important spice / medicinal tree. This investigation details morphoanatomical features of leaf and stem and explains trichome and secretory gland structures that may be associated with medicinal properties of the plant. Transverse sections of leaf and stem clearly reveal position of oil glands (schizolysigenous glands) and other constituent cells. Oil glands are situated close to the epidermis for the release of secretion. The plant body is covered by cuticle, a uniseriate epidermis and is followed by multiple layers of cortex composed of polygonal, starch grains containing parenchymatous cells. Cortex has interspersed oil glands close to the epidermis and few cells of cortex contain calcium oxalate crystals. Inner cortex contains 2-3... layers of sclerenchymatous cells seen as a red band due to safranin staining. In the leaves, oil glands are embedded in the palisade cells near the upper epidermis and the leaf margins exhibit glands opening through a pore covered by wax. Non-glandular trichomes are present on the leaf but with higher density on mid vein. These trichomes have rough surface due to the presence of micropapillae that may function as a deterrent to the insects. Scanning electron microscopy of the trichome cross section reveals central cavity. Fluorescent staining of trichomes for the presence of secondary metabolites was positive using Diphenyl boric acid-β-ethylaminoester stain under UV light. Biochemical assays of M. koenigii leaf extract estimated total polyphenol and total flavonoid contents at 547 mg/g and 401 µmol/g, respectively. Same extract registered a TROLOX Equivalent Antioxidant Capacity of 211 µg/mL.