Modeling the dynamics and kinetics of HIV-1 Gag during viral assembly
作者: Michael D. TomasiniDaniel S. JohnsonJoshua S. MincerSanford M. Simon
作者单位: 11 Laboratory of Cellular Biophysics, Rockefeller University, New York, New York, United States of America
2 2 Department of Physics and Astronomy, Hofstra University, 151 Hofstra University, Hempstead, New York, United States of America
3 3 Department of Anesthesiology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America
刊名: PLOS ONE, 2018, Vol.13 (4)
来源数据库: PLOS
DOI: 10.1371/journal.pone.0196133
原始语种摘要: We report a computational model for the assembly of HIV-1 Gag into immature viral particles at the plasma membrane. To reproduce experimental structural and kinetic properties of assembly, a process occurring on the order of minutes, a coarse-grained representation consisting of a single particle per Gag molecule is developed. The model uses information relating the functional interfaces implicated in Gag assembly, results from cryo electron-tomography, and biophysical measurements from fluorescence microscopy, such as the dynamics of Gag assembly at single virions. These experimental constraints eliminated many classes of potential interactions, and narrowed the model to a single interaction scheme with two non-equivalent interfaces acting to form Gags into a hexamer, and a third...
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来源刊物:
影响因子:3.73 (2012)

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关键词翻译
关键词翻译
  • assembly 组装
  • curvature 曲率
  • kinetics 动力学
  • computational 计算的
  • membrane 
  • single 单独的
  • factor 因素
  • simulation 模拟
  • model 模型
  • spacing 间隔