Genetic characteristics and molecular epidemiology of vancomycin-resistant Enterococci isolates from Caribbean countries
作者: Patrick Eberechi AkpakaShivnarine KissoonPadman JayaratneClyde WilsonGeorge R. GoldingAlison M. NicholsonDelores B. LewisSandra M. HermelijnAlexis Wilson-PearsonAshley Smith
作者单位: 11 The University of the West Indies, Paraclinical Sciences, St. Augustine, Trinidad & Tobago
2 2 McMaster University, Department of Pathological Sciences, Hamilton, Canada
3 3 King Edward VII Memorial Hospital, Department of Pathology and Microbiology, Hamilton, Bermuda
4 4 Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, Canada
5 5 The University of the West Indies, Department of Microbiology, Mona—Kingston, Jamaica
6 6 The University of the West Indies, Faculty of Medical Sciences, Cave Hill, Barbados
7 7 Academic Hospital Paramaribo, Medical Microbiology Laboratory, Paramaribo, Suriname
8 8 Georgetown Public Hospital Corporation, Microbiology Laboratory, Georgetown, Guyana
9 9 King Edward VII Memorial Hospital, Department of Pharmacy, Hamilton, Bermuda
刊名: PLOS ONE, 2017, Vol.12 (10)
来源数据库: PLOS
DOI: 10.1371/journal.pone.0185920
原始语种摘要: Emergence of vancomycin-resistant Enterococci (VRE) that first appeared on the stage about three decades ago is now a major concern worldwide as it has globally reached every continent. Our aim was to simply undertake a multinational study to delineate the resistance and virulence genes of clinical isolates of VRE isolates from the Caribbean. We employed both conventional (standard microbiological methods including use of E-test strips, chromogenic agar) and molecular methods (polymerase chain reactions–PCR, pulsed-field gel electrophoresis–PFGE and multilocus sequence typing–MLST) to analyze and characterize 245 Enterococci species and 77 VRE isolates from twelve hospitals from eight countries in the Caribbean. The PCR confirmed and demonstrated the resistance and virulence genes ( van A...
全文获取路径: PLOS 
影响因子:3.73 (2012)

  • isolates 单离体
  • vancomycin 万古霉素
  • resistant 耐久的
  • PFGE 脉冲场凝胶电泳
  • confirmed 已确认的
  • molecular 分子的
  • virulence 病毒性
  • delineate 描绘
  • polymerase 聚合酶
  • PCR Page Control Register