Genomic analysis of carbapenemase-producing Enterobacteriaceae in Queensland reveals widespread transmission of bla <sub>IMP-4</sub> on an IncHI2 plasmid
作者: Leah W. RobertsElizabeth CatchpooleAmy V. JennisonHaakon BerghAnna HumeClaire HeneyNarelle GeorgeDavid L. PatersonMark A. SchembriScott A. BeatsonPatrick N. A. Harris
作者单位: 1The University of Queensland, Brisbane, QLD , Australia
2Pathology Queensland, QLD , Australia
3Coopers Plains, QLD , Australia
4University of Queensland, Faculty of Medicine, UQ Centre for Clinical Research, Royal Brisbane & Women’s Hospital, QLD , Australia
5The University of Queensland, Brisbane , Australia
刊名: Microbial Genomics, 2019
来源数据库: Microbiology Society
DOI: 10.1099/mgen.0.000321
关键词: CPEIMP-4IncHI2QueenslandGenomicsWhole genome sequencingNanopore
原始语种摘要: Carbapenemase-producing Enterobacteriaceae (CPE) are an increasingly common cause of healthcare-associated infections and may occasionally be identified in patients without extensive healthcare exposure. bla IMP-4 is the most frequently detected carbapenemase gene in Enterobacteriaceae within Australia, but little is known about the mechanisms behind its persistence. Here we used whole genome sequencing (WGS) to investigate the molecular epidemiology of bla IMP-4 in Queensland, Australia. In total, 107 CPE were collected between 2014 and 2017 and sent for WGS on an Illumina NextSeq500. Resistance genes and plasmid types were detected using a combination of read mapping and nucleotide comparison of de novo assemblies. Six isolates were additionally sequenced using...
全文获取路径: 英国微生物学会 

  • plasmid 质粒
  • sequencing 排序
  • nucleotide 核苷酸
  • producing 产油
  • representative 代理的
  • extensive 广泛的
  • isolates 单离体
  • genome 基因组
  • several 各自
  • investigate